DNA Purification

DNA purification is a crucial step in the process of preparing samples. It removes enzymes and salts from the lysed samples or PCR products, before sequencing and cloning. It also eliminates unwanted PCR-induced adversities like primer dimers and nucleotides that are not incorporated. DNA purification in molecular biological research is a crucial step that requires careful planning in order to produce quality, reliable results.

There are a variety of approaches to cleaning DNA. Traditional DNA isolation methods involve many steps including leukocyte isolation or red blood cell lysis for the removal of heme proteins that inhibit the PCR reaction, deproteinization and RNAse treatment, ethanol and isopropanol precipitation. Finally, there is DNA elimination. These procedures require specialized equipment, like an electrophoresis system and biosafety cabinets due to the intercalating dyes that are used in electrophoresis on gels.

Other DNA purification techniques use spin columns or 96-well filter plates to eliminate contaminants by adsorbing them to the surface of the column or plate. These methods can be quite laborious, especially when dealing with large quantities of samples or when the columns need to be manually filled with fresh agents.

Dipsticks significantly reduce the number of steps involved in processing samples to only three. They bind nucleic acids with a waxy substance made of cellulose and then release them in the presence of water. This approach is particularly useful in https://mpsciences.com/ low-resource settings, like remote field sites as well as teaching labs. Its simplicity (30 s per sample) is a great fit for molecular diagnostic tests such as disease detection and genotype screening.

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